Description
Anti-HA Affinity Gel is prepared by conjugating high quality murine IgG1 monoclonal antibody with Sepharose 4B gel. This product has a high HA label fusion protein loading capacity (at least 1.5 mg protein/mL gel) and less non-specific binding of miscellaneous proteins. It can be used for immunoprecipitation (IP) and purification of fusion proteins with HA labels.
Features
- Specific—highly specific monoclonal antibody
- High yield and high purity
- Versatile—It can be used for protein purification or COIP
- Convenient—reagents and peptides to elute are available separately
Applications
- Immunoprecipitation (IP)
- Purification of fusion proteins with HA labels.
Specifications
| Clone | 1A4 |
| Isotype | Mouse IgG1 |
| Purity | Protein A |
| Antibody Concentration | 7.5 g antibody/L Gel |
| Protein binding capacity | ≥1.5 mg protein/mL Gel |
| Storage buffer | 10 mM Na3PO4, 150 mM NaCl, 50% glycerin, pH7.4, contains 0.02% (w/v) sodium azide |
Components
| Components No. | Name | 20586ES03 | 20586ES08 | 20586ES25 | 20586ES60 |
| 20586 | Anti-HA Affinity Gel | 1 mL | 5 mL | 25 mL | 100 mL |
Storage
The unopened products should be stably stored at -25℃ ~ -15℃ for 1 year. Do not freeze in solution without glycerin!
The antibody is clone 1A4, isotype Mouse IgG1, purified by Protein A. Antibody concentration: 7.5 g antibody/L gel. This clone provides high specificity for the HA (YPYDVPDYA) tag with minimal cross-reactivity to endogenous proteins.
Compatible with any protein carrying an N-terminal, C-terminal, or internal HA tag (YPYDVPDYA). Also captures tandem HA tag constructs (2×HA, 3×HA). Does not recognize unrelated epitope tags (Flag, Myc, His, V5).
Equilibrate gel in your lysis/IP buffer (typically 50 mM Tris-HCl pH 7.4–7.5, 150 mM NaCl, 1% NP-40 or 0.5% Triton X-100). Add clarified lysate to the gel and incubate 1–4 h at 4°C with rotation. Wash 3–5× with IP buffer. Elute with HA peptide (1 mg/mL) or pH 2.5–3.0 glycine.
Yes — the gel can be regenerated with low-pH glycine washes followed by re-equilibration. However, the number of cycles before significant performance loss depends on sample complexity and handling. Store regenerated gel in 10 mM Na₃PO₄, 150 mM NaCl, 50% glycerol, pH 7.4, 0.02% sodium azide at 2–8°C. Do not freeze.
Unopened product: store at −25°C to −15°C for 1 year. Do not freeze without the supplied glycerol-containing storage buffer. After opening, aliquot and store at 2–8°C to avoid repeated freeze-thaw. Avoid contamination.
The following customization and add-on services may be available for this product type through the supplier. For inquiries and pricing, contact support@biohippo.com.
Customization Options
- Bulk Quantities: Larger volumes and custom pack sizes beyond the standard catalog may be available for high-throughput core facilities and industrial customers. Contact us to inquire about bulk pricing.
- Custom Antibody Coupling (Magnetic Beads): Custom magnetic beads conjugated to your specific antibody or protein ligand may be available through Yeasen's OEM and custom services program. These services leverage the same bead platform used for catalog products.
- OEM Manufacturing: Custom bead formulations for diagnostic device development, including magnetic beads for nucleic acid isolation, IP-grade beads, and affinity capture beads, may be available at manufacturing scale.
To inquire about custom options or bulk pricing, contact support@biohippo.com.
