Anti-HA-Tag Rabbit Monoclonal Antibody

SKU:BHA21008258
Research Validated
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Overview
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Anti-HA-Tag antibody (Rabbit monoclonal, clone BC-8, Rabbit IgG). Validated for Western blot. Reactivity: Human, Mouse, Rat. Recognizes HA tag. Observed band in Western blot: about 15 kDa.
Target HA-Tag
clone number BC-8
Host Rabbit
Reactivity Human,Mouse,Rat
Isotype Rabbit IgG
Application(s) WB
Options selector
Catalog no. Size Conjugation
MT0012 100 uL/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • Size: 100 uL/vial; Conjugation: Unconjugated
      Form: Liquid
      Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
      Applications: WB
      Application details: WB 1:1000-5000<br>
      Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
  • Lead time: typically ships in ~2-3 business days; timing may vary by selected option.
  • Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target HA-Tag
Alternative names Red fluorescent protein eqFP611;GFP-like chromoprotein;
UniProt # P68431
Host Rabbit
Clone BC-8
Clonality
  • Monoclonal
Isotype
  • Rabbit IgG
Reactivity
  • Human
  • Mouse
  • Rat
Applications
  • Western Blot
Immunogen A synthesized peptide
Molecular weight 15.4 kDa
Purification Affinity-chromatography
Cellular localization Lateral cell membrane ; Single-pass type I membrane protein . Cell junction, tight junction . Colocalizes with CLDN7 at the lateral cell membrane and tight junction. .
Concentration 0.5mg/ml
Form Liquid
Storage Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) MT0012
Main SKU BHA21008258
Rabbit Monoclonal

Product Overview

Clone BC-8 is a rabbit monoclonal antibody that recognizes the HA tag and is validated for Western blot. The predicted molecular weight is 15.4 kDa, and the supplier reports an observed band at about 15 kDa.

Target HA tag
Clone BC-8
Host / Clonality / Isotype Rabbit / Monoclonal / Rabbit IgG
Reactivity Human, Mouse, Rat
Form Liquid
Formulation PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers
Calculated MW 15.4 kDa
Observed MW 15 kDa
Storage Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Validated Applications

Western blot 1:1000–1:5000

Samples with a confirmed band (WB): HA-tag fusion protein.

Recommended loading (WB): 20–40 µg of total protein per lane.

Immunogen

A synthesized peptide.

Reactivity Notes

This antibody detects the HA tag only on proteins that carry it; it does not recognize an endogenous protein.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: Western blot 1:1000–1:5000. Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Human, Mouse, Rat. Other species are not listed by the supplier.
Q.What band size should be expected in Western blot?
A.Reported by the supplier: calculated molecular weight 15.4 kDa; observed band at about 15 kDa.
Q.What immunogen was used to raise this antibody?
A.A synthesized peptide.
Q.How should this antibody be stored?
A.Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

Lizhi Gong et al. (2026) Discovery of Hirsutellones as Nucleolin-Targeting Natural Macrocycles for Triple-Negative Breast Cancer Treatment. Journal of Medicinal Chemistry. 10.1021/acs.jmedchem.6c00021

Shuwei Peng et al. (2021) Myricetin exerts its antiviral activity against infectious bronchitis virus by inhibiting the deubiquitinating activity of papain-like protease. Poultry Science. 10.1016/j.psj.2021.101626

Hou-Zhen Shi et al. (2024) The potential benefits of PGC-1α in treating Alzheimer's disease are dependent on the integrity of the LLKYL L3 motif: Effect of regulating mitochondrial axonal transportation. Experimental Gerontology. 10.1016/j.exger.2024.112514

Min Sun et al. (2021) Screening interferon antagonists from accessory proteins encoded by P gene for immune escape of Caprine parainfluenza virus 3. Veterinary Microbiology. 10.1016/j.vetmic.2021.108980

Liu-gang TAN et al. (2019) Glycosylation of the hemagglutinin protein of H9N2 subtype avian influenza virus influences its replication and virulence in mice. Journal of Integrative Agriculture. 10.1016/S2095-3119(19)62669-9

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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

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