| Field | Specification |
|---|---|
| Target | |
| Alternative names | 3-hydroxyanthranilate 3,4-dioxygenase; 3-hydroxyanthranilate oxygenase; 3-HAO; h3HAO; 3-hydroxyanthranilic acid dioxygenase; HAD; HAAO |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human HAAO recombinant protein (Position: E52-G286). Human HAAO shares 86.3% and 88.5% amino acid (aa) sequence identity with mouse and rat HAAO, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets 3-hydroxyanthranilate 3,4-dioxygenase (gene HAAO) in human and rat samples and is validated for Western blot, IHC and ELISA. The predicted molecular weight is 32.6 kDa, and the supplier reports an observed band at about 33 kDa.
3-Hydroxyanthranilate 3,4-dioxygenase (HAAO) is an iron-dependent enzyme of the kynurenine pathway of tryptophan breakdown. It opens the ring of 3-hydroxyanthranilate, and the product cyclizes to quinolinic acid, a precursor for de novo NAD+ synthesis and a known neuroactive metabolite. HAAO is studied in tryptophan metabolism, NAD+ biology and neuroinflammation.
| Target | 3-hydroxyanthranilate 3,4-dioxygenase (gene HAAO; UniProt P46952, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 32.6 kDa |
| Observed MW | 33 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HepG2 cells, human THP-1 cells, human HCCP cells, rat liver tissue, mouse liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human liver cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human HAAO fragment (Glu52–Gly286), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 86.3% and 88.5%, respectively.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places 3-hydroxyanthranilate 3,4-dioxygenase in the cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
