| Field | Specification |
|---|---|
| Target | |
| Alternative names | Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial; HCDH; Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase; Short-chain 3-hydroxyacyl-CoA dehydrogenase; HADH; HAD; HAD1; HADHSC; SCHAD |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human HADH recombinant protein (Position: A43-K301). Human HADH shares 90.3% and 90.7% amino acid (aa) sequence identity with mouse and rat HADH, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes hydroxyacyl-coenzyme A dehydrogenase, mitochondrial (gene HADH) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 34.3 kDa, and the supplier reports an observed band at about 34 kDa.
Hydroxyacyl-CoA dehydrogenase (HADH) is a mitochondrial enzyme that carries out the third step of fatty acid β-oxidation for short- and medium-chain substrates. In pancreatic β-cells it also restrains glutamate dehydrogenase, limiting amino acid-stimulated insulin release, and its deficiency causes congenital hyperinsulinism. HADH is studied in fatty acid oxidation and insulin secretion.
| Target | Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial (gene HADH; UniProt Q16836, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 34.3 kDa |
| Observed MW | 34 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Rat) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HepG2 cells, human Jurkat cells, human 293T cells, human A431 cells, rat liver tissue, rat kidney tissue, mouse liver tissue, mouse kidney tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.25 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human colon adenocarcinoma tissue, human glioblastoma tissue, human liver cancer tissue, human lung adenocarcinoma tissue, human pancreas ductal adenocarcinoma tissue, human testicular seminoma tissue, human tonsil tissue, human urothelial carcinoma tissue, rat alcohol liver tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): HeLa cells.
Samples with confirmed staining (ICC/IF): human intestinal cancer tissue.
Samples with a confirmed signal (flow cytometry): HepG2 cells.
Immunogen
Recombinant human HADH fragment (Ala43–Lys301), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 90.3% and 90.7%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places hydroxyacyl-coenzyme A dehydrogenase, mitochondrial in the mitochondrion matrix. Tissue expression noted by UniProt (human): Expressed in liver, kidney, pancreas, heart and skeletal muscle. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
