| Field | Specification |
|---|---|
| Target | |
| Alternative names | Trifunctional enzyme subunit alpha, mitochondrial; 78 kDa gastrin-binding protein; Monolysocardiolipin acyltransferase; MLCL AT; TP-alpha; HADHA; HADH |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human HADHA recombinant protein (Position: R20-N758). Human HADHA shares 86.6% and 95.2% amino acid (aa) sequence identity with mouse and rat HADHA, respectively. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets trifunctional enzyme subunit alpha, mitochondrial (gene HADHA) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, flow cytometry and ELISA.
Trifunctional enzyme subunit alpha (HADHA) is part of the mitochondrial trifunctional protein, which carries out the last three steps of β-oxidation for long-chain fatty acids. The alpha subunit provides the enoyl-CoA hydratase and 3-hydroxyacyl-CoA dehydrogenase activities. Inherited HADHA defects cause LCHAD and trifunctional protein deficiencies, and it is studied in fatty acid metabolism and cardiolipin remodeling.
| Target | Trifunctional enzyme subunit alpha, mitochondrial (gene HADHA; UniProt P40939, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
| Immunocytochemistry / Immunofluorescence | Not specified by the supplier |
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human HADHA fragment (Arg20–Asn758), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 86.6% and 95.2%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places trifunctional enzyme subunit alpha, mitochondrial in the mitochondrion and mitochondrion inner membrane.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
