| Field | Specification |
|---|---|
| Target | |
| Alternative names | Ubiquitin C-terminal hydrolase 7; Deubiquitinating enzyme 7; Herpesvirus-associated ubiquitin-specific protease; Ubiquitin thioesterase 7; Ubiquitin-specific-processing protease 7; USP7; HAUSP |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human HAUSP / USP7 Hydrolase that deubiquitinates target proteins such as FOXO4, p53/TP53, MDM2, ERCC6, DNMT1, UHRF1, PTEN and DAXX (PubMed:11923872, PubMed:15053880, PubMed:16964248, PubMed:18716620, PubMed:25283148) . |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AEBC-21 is a rabbit monoclonal antibody that recognizes ubiquitin C-terminal hydrolase 7 (gene USP7) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 128.3 kDa, and the supplier reports an observed band at about 128 kDa.
USP7 (HAUSP) is a deubiquitinating enzyme with many substrates, most famously MDM2 and p53. By stabilizing MDM2 it indirectly promotes p53 degradation, while it can also directly protect p53, placing USP7 at the center of the p53 regulatory circuit. It also regulates DNMT1, UHRF1, PTEN and FOXO proteins and is a studied target in cancer research.
| Target | Ubiquitin C-terminal hydrolase 7 (gene USP7; UniProt Q93009, human) |
|---|---|
| Clone | AEBC-21 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 128.3 kDa |
| Observed MW | 128 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human HAUSP / USP7 Hydrolase that deubiquitinates target proteins such as FOXO4, p53/TP53, MDM2, ERCC6, DNMT1, UHRF1, PTEN and DAXX (PubMed:11923872, PubMed:15053880, PubMed:16964248, PubMed:18716620, PubMed:25283148).
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places ubiquitin C-terminal hydrolase 7 in the nucleus, cytoplasm and chromosome. Tissue expression noted by UniProt (human): Expressed in neural progenitor cells (at protein level).
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Jianming Zhou et al. (2025) CYLD inhibits ubiquitin-mediated degradation of IRE1 to promote inflammatory injury in ulcerative colitis. International Immunopharmacology. 10.1016/j.intimp.2025.115698
