Anti-HB9/HLXB9 Monoclonal Antibody

SKU:BHA21008906
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Overview
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Anti-MNX1 antibody (Rabbit monoclonal, clone ACIE-13, Rabbit IgG). Validated for Western blot and immunoprecipitation. Reactivity: Human, Mouse. Recognizes motor neuron and pancreas homeobox protein 1.
Target MNX1
clone number ACIE-13
Host Rabbit
Reactivity Human,Mouse
Isotype Rabbit IgG
Application(s) WB, IP
Options selector
Catalog no. Size Conjugation
M04520 100 uL/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • Size: 100 uL/vial; Conjugation: Unconjugated
      Form: Liquid
      Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
      Applications: WB,IP
      Application details: WB 1:500-2000<br>IP 1:20<br>
      Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
  • Lead time: typically ships in ~2-3 business days; timing may vary by selected option.
  • Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target MNX1
Alternative names Motor neuron and pancreas homeobox protein 1; Homeobox protein HB9; MNX1; HLXB9
Gene ID 3110
UniProt # P50219
Host Rabbit
Clone ACIE-13
Clonality
  • Monoclonal
Isotype
  • Rabbit IgG
Reactivity
  • Human
  • Mouse
Applications
  • Western Blot
  • Immunoprecipitation
Immunogen A synthesized peptide derived from human HB9/HLXB9
Molecular weight 40.6 kDa
Purification Affinity-chromatography
Reconstitution Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Cellular localization Nucleus.
Concentration 0.5mg/ml
Form Liquid
Storage Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) M04520
Main SKU BHA21008906
Rabbit Monoclonal

Product Overview

Clone ACIE-13 is a rabbit monoclonal antibody that recognizes motor neuron and pancreas homeobox protein 1 (gene MNX1) in human and mouse samples and is validated for Western blot and immunoprecipitation. The predicted molecular weight is 40.6 kDa, and the supplier reports an observed band at about 41 kDa.

MNX1 (also known as HB9 or HLXB9) is a homeodomain transcription factor. It helps establish motor neuron identity in the developing spinal cord and is involved in pancreas development and β-cell function, and it is widely used as a motor neuron marker. Mutations in MNX1 cause Currarino syndrome.

Target Motor neuron and pancreas homeobox protein 1 (gene MNX1; UniProt P50219, human)
Clone ACIE-13
Host / Clonality / Isotype Rabbit / Monoclonal / Rabbit IgG
Reactivity Human, Mouse
Form Liquid
Formulation PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers
Calculated MW 40.6 kDa
Observed MW 41 kDa
Storage Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Validated Applications

Western blot 1:500–1:2000
Immunoprecipitation 1:20

Samples with a confirmed band (WB): Molt-4 cells.

Recommended loading (WB): 20–40 µg of total protein per lane.

Immunogen

Synthetic peptide based on the sequence of human HB9/HLXB9.

Reactivity Notes

The supplier lists reactivity with human and mouse. UniProt places motor neuron and pancreas homeobox protein 1 in the nucleus. Tissue expression noted by UniProt (human): Expressed in lymphoid and pancreatic tissues. The samples tested by the supplier (listed above) are a practical starting point for positive controls.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: Western blot 1:500–1:2000; Immunoprecipitation 1:20. Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Human, Mouse. Other species are not listed by the supplier.
Q.What band size should be expected in Western blot?
A.Reported by the supplier: calculated molecular weight 40.6 kDa; observed band at about 41 kDa.
Q.What immunogen was used to raise this antibody?
A.Synthetic peptide based on the sequence of human HB9/HLXB9.
Q.How should this antibody be stored?
A.Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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