| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Hemoglobin subunit delta; Delta-globin; Hemoglobin delta chain; HBD; |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human HBD recombinant protein (Position: M1-H147). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-HBD Antibody Picoband® is an antibody for HBD detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: HBD (hemoglobin subunit delta); UniProt: P02042
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 16 kDa
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-HBD Antibody Picoband® catalog # A01076-1.
Biological background
Biological context: Involved in oxygen transport from the lung to the various peripheral tissues.
Expression and localization notes: tissue context: Red blood cells..
Common research applications
- Western blotting (WB): Compare HBD levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of HBD in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify HBD-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: Hemoglobin subunit delta is a protein that in humans is encoded by the HBD gene. The delta (HBD) and beta (HBB) genes are normally expressed in the adult: two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of the total hemoglobin. Two alpha chains plus two delta chains constitute HbA-2, which with HbF comprises the remaining 3% of adult hemoglobin. Five beta-like globin genes are found within a 45 kb cluster on chromosome 11 in the following order: 5'-epsilon--Ggamma--Agamma--delta--beta-3'.
- Cross reactivity: No cross-reactivity with other proteins.
- Tissue details: Red blood cells.
- Research category: Biochemicals,Chemical Type,Protein Phosphorylation,Ser/Thr Kinases,Signal Transduction
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
