| Field | Specification |
|---|---|
| Target | |
| Alternative names | Lutropin-choriogonadotropic hormone receptor;LH/CG-R;Luteinizing hormone receptor;LHR;LSH-R;LHCGR;LCGR, LGR2, LHRHR; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human hCG receptor, different from the relative rat sequence by two amino acids, mouse sequence by three amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects lutropin-choriogonadotropic hormone receptor (gene LHCGR) in human and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 78.6 kDa, and the supplier reports an observed band at about 78 kDa.
The LH/choriogonadotropin receptor (LHCGR) is a G protein-coupled receptor that binds luteinizing hormone and human chorionic gonadotropin. Through Gs and cAMP it drives testosterone production in Leydig cells and ovulation and progesterone production in the ovary. LHCGR mutations cause disorders of sexual development and puberty.
| Target | Lutropin-choriogonadotropic hormone receptor (gene LHCGR; UniProt P22888, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg thimerosal; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 78.6 kDa |
| Observed MW | 78 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Rat, Human) |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Human, Rat) |
Samples with a confirmed band (WB): MCF-7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): rat ovary tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide from the N-terminal region of human hCG receptor.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places lutropin-choriogonadotropic hormone receptor in the cell membrane. Tissue expression noted by UniProt (human): Gonadal and thyroid cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Lai Fang-Nong et al. (2016) Di (2-ethylhexyl) phthalate impairs steroidogenesis in ovarian follicular cells of prepuberal mice. Archives of Toxicology. 10.1007/s00204-016-1790-z
Lu Xuefeng et al. (2019) Pregnancy and Live Birth In Women With Pathogenic LHCGR Variants Using Their Own Oocytes. Journal of Clinical Endocrinology & Metabolism. 10.1210/jc.2019-01276
Castinetti Frederic et al. (2023) HCG-responsive aldosteronoma with transient secretion during pregnancy confirmed through HCG-stimulated adrenal venous sampling. Frontiers in Endocrinology. 10.3389/fendo.2023.1153374
Prasanthi P. Koganti et al. (2022) Exogenous cholesterol acquisition signaling in LH-responsive MA-10 Leydig cells and in adult mice. Journal of Endocrinology. 10.1530/joe-22-0043
Jun-Jie Xu et al. (2019) Role of nuclear factor-κB pathway in the transition of mouse secondary follicles to antral follicles. Journal of Cellular Physiology. 10.1002/jcp.28822
Xin Luo et al. (2021) The Role of Inactivated NF-κB in Premature Ovarian Failure. American Journal of Pathology. 10.1016/j.ajpath.2021.12.005
Wen Xiaowei et al. (2018) The role of combining medroxyprogesterone 17-acetate with human menopausal gonadotropin in mouse ovarian follicular development. Scientific Reports. 10.1038/s41598-018-22797-6
Cong Liu et al. (2013) Female hormone release of microencapsulated Xenopus laevis ovarian cells. International Journal of Pharmaceutics. 10.1016/j.ijpharm.2013.04.048
H.S. Dong et al. (2009) Premeiotic fetal murine germ cells cultured in vitro form typical oocyte-like cells but do not progress through meiosis. Theriogenology. 10.1016/j.theriogenology.2009.02.021
Yaping Ren et al. (2019) Mechanism of cadmium poisoning on testicular injury in mice. Oncology Letters. 10.3892/ol.2019.10418
