| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1;Brain cyclic nucleotide-gated channel 1;BCNG-1;HCN1;BCNG1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human HCN1 recombinant protein (Position: E618-L890). Human HCN1 shares 82% amino acid (aa) sequence identity with both mouse and rat HCN1. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of HCN1 (Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-HCN1 Antibody Picoband® catalog # PB9210. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E.coli-derived human HCN1 recombinant protein (Position: E618-L890). Human HCN1 shares 82% amino acid (aa) sequence identity with both mouse and rat HCN1. (reported region: E618-L890).
- Molecular weight context: reported MW: 120 kDa; calculated MW: 98796 MW
- Reactivity: Human,Mouse,Rat
- Applications: WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1; Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1. Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1, also known as HAC-2 or BCNG-1, is a protein that in humans is encoded by the HCN1 gene. It is mapped to 5p12. The membrane protein encoded by this gene is a hyperpolarization-activated cation channel that contributes to the native pacemaker currents in heart and neurons. The encoded protein can homodimerize or heterodimerize with other pore-forming subunits to form a potassium channel. This channel may act as a receptor for sour tastes. Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. It may mediates responses to sour stimuli. Functional note: Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). May mediate responses to sour stimuli. . Reported localization: Cell membrane ; Multi-pass membrane protein . Expression/tissue context: Detected in brain, in particular in amygdala and hippocampus, while expression in caudate nucleus, corpus callosum, substantia nigra, subthalamic nucleus and thalamus is very low or not detectable. Detected at very low levels in muscle and pancreas. .
Research relevance and current trends
- Cardiovascular: Researchers commonly examine how HCN1 (Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1) relates to this theme using model systems and orthogonal readouts.
- Contractility: Researchers commonly examine how HCN1 (Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1) relates to this theme using model systems and orthogonal readouts.
- Neuroscience: Researchers commonly examine how HCN1 (Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative HCN1 (Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1) levels across conditions; band patterns may reflect isoforms and processing.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Family / similarity context: Belongs to the potassium channel HCN family.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
