| Field | Specification |
|---|---|
| Target | |
| Alternative names | Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2;Brain cyclic nucleotide-gated channel 2;BCNG-2;HCN2;BCNG2; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human HCN2, identical to the related mouse sequence, and different from the related rat sequence by one amino acid. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 (gene HCN2) in human, mouse and rat samples and is validated for IHC and Western blot. The predicted molecular weight is 97.0 kDa, and the supplier reports an observed band at about 97 kDa.
HCN2 is a hyperpolarization-activated cyclic nucleotide-gated channel that passes Na+ and K+ and is strongly modulated by cAMP. It contributes to pacemaker currents in the heart (If) and neurons (Ih) and has been implicated in the onset of neuropathic pain in sensory neurons. HCN2 is studied in neuronal excitability, cardiac rhythm and pain.
| Target | Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 (gene HCN2; UniProt Q9UL51, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 97.0 kDa |
| Observed MW | 97 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Rat, Human) |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Mouse, Rat, Human) |
Samples with a confirmed band (WB): rat brain tissue, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): rat brain tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Immunogen
Synthetic peptide from the C-terminal region of human HCN2. The peptide sequence is conserved in mouse. It differs from the rat sequence at one residue.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 in the cell membrane. Tissue expression noted by UniProt (human): Highly expressed throughout the brain. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Ze-Jun Wang et al. (2020) The HCN domain is required for HCN channel cell-surface expression and couples voltage- and cAMP-dependent gating mechanisms. Journal of Biological Chemistry. 10.1074/jbc.RA120.013281
Shuang Xia et al. (2010) Dynamic changes in HCN2, HCN4, KCNE1, and KCNE2 expression in ventricular cells from acute myocardial infarction rat hearts. Biochemical and Biophysical Research Communications. 10.1016/j.bbrc.2010.04.003
Tao Song et al. (2011) Spironolactone diminishes spontaneous ventricular premature beats by reducing HCN4 protein expression in rats with myocardial infarction. Molecular Medicine Reports. 10.3892/mmr.2011.455
