| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2;Brain cyclic nucleotide-gated channel 2;BCNG-2;HCN2;BCNG2; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human HCN2, identical to the related mouse sequence, and different from the related rat sequence by one amino acid. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of HCN2 (Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-HCN2 Antibody Picoband® catalog # A02804. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence at the C-terminus of human HCN2, identical to the related mouse sequence, and different from the related rat sequence by one amino acid.
- Molecular weight context: reported MW: 97 kDa; calculated MW: 96950 MW
- Reactivity: Human,Mouse,Rat
- Applications: IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2; Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2. Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated ion channel 2 is a protein that in humans is encoded by the HCN2 gene. The HCN2 gene is localized on human chromosome 19p13.3 and contains eight exons spanning approximately 27 kb. Hyperpolarization-activated cation channels of the HCN gene family, such as HCN2, contribute to spontaneous rhythmic activity in both heart and brain. Functional note: Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Can also transport ammonium in the distal nephron. Produces a large instantaneous current. Modulated by intracellular chloride ions and pH; acidic pH shifts the activation to more negative voltages (By similarity). . Reported localization: Cell membrane ; Multi-pass membrane protein . Expression/tissue context: Highly expressed throughout the brain. Detected at low levels in heart. .
Research relevance and current trends
- Cardiovascular: Researchers commonly examine how HCN2 (Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2) relates to this theme using model systems and orthogonal readouts.
- Contractility: Researchers commonly examine how HCN2 (Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2) relates to this theme using model systems and orthogonal readouts.
- Neuroscience: Researchers commonly examine how HCN2 (Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative HCN2 (Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of HCN2 (Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2) across tissue regions and cell types using matched controls.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
