| Field | Specification |
|---|---|
| Target | |
| Alternative names | Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2; Brain cyclic nucleotide-gated channel 2; BCNG-2; Hyperpolarization-activated cation channel 1; HAC-1; Hcn2; Bcng2; Hac1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived mouse Hcn2 recombinant protein (Position: E660-L863). Mouse Hcn2 shares 87.4% and 97.5% amino acid (aa) sequence identity with human and rat HCN2, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 (gene Hcn2) in mouse and rat samples and is validated for Western blot, IHC, flow cytometry and ELISA. The predicted molecular weight is 94.7 kDa, and the supplier reports an observed band at about 110 kDa.
HCN2 is a hyperpolarization-activated cyclic nucleotide-gated channel that passes Na+ and K+ and is strongly modulated by cAMP. It contributes to pacemaker currents in the heart (If) and neurons (Ih) and has been implicated in the onset of neuropathic pain in sensory neurons. HCN2 is studied in neuronal excitability, cardiac rhythm and pain.
| Target | Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 (gene Hcn2; UniProt O88703, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 94.7 kDa |
| Observed MW | 110 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Mouse, Rat) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Mouse) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): rat brain tissue, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse brain tissue, mouse cardiac tissue, rat brain tissue, rat cardiac tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with a confirmed signal (flow cytometry): Neuro-2a cells, NIH/3T3 cells.
Immunogen
Recombinant mouse Hcn2 fragment (Glu660–Leu863), expressed in E. coli. Sequence identity with the human and rat orthologs: 87.4% and 97.5%, respectively.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 in the cell membrane. Tissue expression noted by UniProt (mouse): Highly expressed in brain. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
