| Field | Specification |
|---|---|
| Target | |
| Alternative names | Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4; HCN4 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human HCN4 recombinant protein (Position: H909-L1203). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4 (gene HCN4) in human, mouse and rat samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 129.0 kDa, and the supplier reports an observed band at about 140 kDa.
HCN4 is the main hyperpolarization-activated cyclic nucleotide-gated channel of the sinoatrial node, where it carries the funny current (If) that sets heart rate. It activates slowly on hyperpolarization and is enhanced by cAMP, linking heart rate to sympathetic input. HCN4 variants cause sinus node dysfunction, and it is the target of the heart-rate-lowering drug ivabradine.
| Target | Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4 (gene HCN4; UniProt Q9Y3Q4, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 129.0 kDa |
| Observed MW | 140 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): rat brain tissue, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): MCF-7 cells.
Immunogen
Recombinant human HCN4 fragment (His909–Leu1203), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4 in the cell membrane. Tissue expression noted by UniProt (human): Highly expressed in thalamus, testis and in heart, both in ventricle and atrium. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Lei Yang et al. (2025) HCN2 promotes neurodevelopmental and synaptic function repair through the CaMKII/CREB pathway to alleviate general anesthesia-induced cognitive impairment. Cellular Signalling. 10.1016/j.cellsig.2025.112113
Shuang Xia et al. (2010) Dynamic changes in HCN2, HCN4, KCNE1, and KCNE2 expression in ventricular cells from acute myocardial infarction rat hearts. Biochemical and Biophysical Research Communications. 10.1016/j.bbrc.2010.04.003
Du Jianlin et al. (2017) Age-dependent down-regulation of hyperpolarization-activated cyclic nucleotide-gated channel 4 causes deterioration of canine sinoatrial node function. ACTA Biochimica et Biophysica Sinica. 10.1093/abbs/gmx026
Tao Song et al. (2011) Spironolactone diminishes spontaneous ventricular premature beats by reducing HCN4 protein expression in rats with myocardial infarction. Molecular Medicine Reports. 10.3892/mmr.2011.455
