| Field | Specification |
|---|---|
| Target | |
| Alternative names | Hypocretin neuropeptide precursor; Hypocretin; Hcrt; Orexin precursor; Prepro-orexin; Preprohypocretin; OX; PPORX; PPOX |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human HCRT, identical to the related mouse and rat sequences. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets hypocretin neuropeptide precursor (gene HCRT) in mouse and rat samples and is validated for IHC and ICC/IF. The predicted molecular weight is 13.4 kDa.
The hypocretin precursor (HCRT) is processed into two neuropeptides, orexin-A and orexin-B, made by a small group of neurons in the lateral hypothalamus. Acting on the OX1R and OX2R receptors, they promote wakefulness and regulate feeding and energy balance. Loss of orexin neurons causes narcolepsy with cataplexy, and HCRT is studied in sleep and metabolism.
| Target | Hypocretin neuropeptide precursor (gene HCRT; UniProt O43612, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 13.4 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 2–5 µg/mL (Mouse, Rat) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Mouse, Rat) |
Samples with confirmed staining (IHC): mouse brain tissue, rat brain tissue, rat cerebellum tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): mouse brain tissue, rat brain tissue.
Immunogen
Synthetic peptide from the middle region of human HCRT. The peptide sequence is conserved in mouse and rat.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places hypocretin neuropeptide precursor in the rough endoplasmic reticulum, cytoplasmic vesicle and synapse. Tissue expression noted by UniProt (human): Abundantly expressed in subthalamic nucleus but undetectable in other brain regions tested (hypothalamus was not tested) and in heart, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
