| Field | Specification |
|---|---|
| Target | |
| Alternative names | Histone deacetylase 1;HD1;3.5.1.98;HDAC1;RPD3L1; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human HDAC1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AIB-8 is a rabbit monoclonal antibody that recognizes histone deacetylase 1 (gene HDAC1) in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 55.1 kDa, and the supplier reports an observed band at about 55–60 kDa.
Histone deacetylase 1 (HDAC1) is a class I deacetylase that removes acetyl groups from lysines on core histones and other proteins. Removing acetyl groups from histone tails generally compacts chromatin and represses transcription. It works within corepressor complexes such as Sin3, NuRD and CoREST, often with HDAC2, and is studied in gene regulation, development and cancer.
| Target | Histone deacetylase 1 (gene HDAC1; UniProt Q13547, human) |
|---|---|
| Clone | AIB-8 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 55.1 kDa |
| Observed MW | 55–60 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:1000–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with confirmed staining (IHC): human testis tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human HDAC1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places histone deacetylase 1 in the nucleus. Tissue expression noted by UniProt (human): Ubiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
