| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Bone morphogenetic protein receptor type-2; BMP type-2 receptor; BMPR-2; Bone morphogenetic protein receptor type II; BMP type II receptor; BMPR-II; BMPR2; PPH1 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human HDAC2 recombinant protein (Position: E387-P488). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-HDAC2 Antibody Picoband® is an antibody for HDAC2 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, IF, ICC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: HDAC2 (bone morphogenetic protein receptor type 2); UniProt: Q92769
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 60 kDa, calculated 29041 MW
- Applications: WB, IHC, IF, ICC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-HDAC2 Antibody Picoband® catalog # A00325-3.
Biological background
Biological context: On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Binds to BMP7, BMP2 and, less efficiently, BMP4. Binding is weak but enhanced by the presence of type I receptors for BMPs. Mediates induction of adipogenesis by GDF6.
Expression and localization notes: cellular localization: Cell membrane., tissue context: Highly expressed in heart and liver..
Common research applications
- Western blotting (WB): Compare HDAC2 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of HDAC2 in tissue sections, considering fixation and antigen retrieval effects.
- Immunofluorescence / ICC: Assess subcellular localization patterns and co-localization with compartment markers in cultured cells.
- Flow cytometry: Quantify HDAC2-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: Histone deacetylase 2 is an enzyme that in humans is encoded by the HDAC2 gene. This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes and are responsible for the deacetylation of lysine residues on the N-terminal region of the core histones (H2A, H2B, H3 and H4). This protein also forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Betz et al. (1998) performed PCR using HDAC2-specific primers to screen a somatic cell hybrid mapping panel. They mapped the HDAC2 gene to human chromosome 6q21, a region of the genome altered in some cancers, including retinoblastoma.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Cell membrane.
- Tissue details: Highly expressed in heart and liver.
- Research category: Cardiovascular,Cytoskeleton/ECM,Extracellular Matrix,Growth Factors/Hormones,Hypertrophy,Mesenchymal Stem Cells,Protein Phosphorylation,Ser/Thr Kinases,Signal Transduction,Signaling Pathways,Stem Cells,Structures,Surface Molecules,TGF Beta
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
