| Field | Specification |
|---|---|
| Target | |
| Alternative names | Histone deacetylase 3;HD3;3.5.1.98;RPD3-2;SMAP45;HDAC3; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human HDAC3 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone EFH-8 is a rabbit monoclonal antibody that recognizes histone deacetylase 3 (gene HDAC3) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and immunoprecipitation. The predicted molecular weight is 48.8 kDa, and the supplier reports an observed band at about 49 kDa.
Histone deacetylase 3 (HDAC3) is a class I deacetylase that forms the catalytic core of the NCoR/SMRT corepressor complex, which needs HDAC3 for activity. Removing acetyl groups from histone tails generally compacts chromatin and represses transcription. HDAC3 is studied in metabolism, circadian gene regulation, inflammation and development.
| Target | Histone deacetylase 3 (gene HDAC3; UniProt O15379, human) |
|---|---|
| Clone | EFH-8 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 48.8 kDa |
| Observed MW | 49 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:30 |
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:1000 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human HDAC3.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places histone deacetylase 3 in the nucleus, chromosome and cytoplasm. Tissue expression noted by UniProt (human): Widely expressed.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Zeng Chong et al. (2025) Inhibition of histone deacetylases 3 attenuates imiquimod-induced psoriatic dermatitis via targeting cGAS-STING signaling in keratinocytes. Journal of Translational Medicine. 10.1186/s12967-025-06544-w
