| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Histone deacetylase 4;HD4;3.5.1.98;HDAC4;KIAA0288; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthesized peptide derived from human HDAC4 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-HDAC4/Histone Deacetylase 4 Rabbit Monoclonal Antibody is an antibody targeting HDAC4. Common applications include WB, ICC, IF. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: HGI-8; isotype: Rabbit IgG; reactivity: Human; observed MW: 180-230 kDa; calculated MW: 119040 MW.
Boster Bio Anti-HDAC4/Histone Deacetylase 4 Rabbit Monoclonal Antibody catalog # M00971. Tested in WB, ICC/IF applications. This antibody reacts with Human.
Key elements and design rationale
- Target: HDAC4 — Histone deacetylase 4
- Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: HGI-8; Isotype: Rabbit IgG
- Species reactivity: Human
- Molecular weight guidance: Observed: 180-230 kDa; Calculated: 119040 MW
Biological background
Protein function (datasheet): Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. .
Cellular localization (datasheet): Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. Upon muscle cells differentiation, it accumulates in the nuclei of myotubes, suggesting a positive role of nuclear HDAC4 in muscle differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-246, Ser-467 and Ser-632 by CaMK4 and SIK1. The nuclear localization probably depends on sumoylation.
Tissue details (datasheet): Ubiquitous.
Research relevance and current trends
- Commonly studied in contexts related to Acetylation,Cardiovascular,Chromatin Modifying Enzymes,Epigenetics and Nuclear Signaling,Hypertrophy,Signaling Pathways,Stem Cells.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
