Anti-HDAC7/Histone Deacetylase 7 Rabbit Monoclonal Antibody

SKU:BHA21008146
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Suppliers
Boster Bio
Boster Bio
Details Products
Overview
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Anti-HDAC7 antibody (Rabbit monoclonal, clone HFG-8, Rabbit IgG). Validated for Western blot and flow cytometry. Reactivity: Human, Mouse, Rat. Recognizes histone deacetylase 7. Observed band in Western blot: about 124 kDa.
Target HDAC7
Clone number HFG-8
Host Rabbit
Reactivity Human,Mouse,Rat
Isotype Rabbit IgG
Application(s) WB, Flow Cytometry
Options selector
Catalog no. Size Conjugation
M01913 100 uL/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • 100 uL/vial — Unconjugated
      Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
      Form: Liquid
      Applications: WB,Flow Cytometry
      Application details: WB 1:500-2000
      FC 1:20
      Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
  • Lead time: typically ships in 2-3 business days; timing may vary by selected option.
  • Storage: refer to the option details above and the product datasheet for storage and handling.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible; avoid repeated freeze-thaw cycles.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target HDAC7
Alternative names Histone deacetylase 7;HD7;3.5.1.98;Histone deacetylase 7A;HD7a;HDAC7;HDAC7A;
UniProt # Q8WUI4
Host Rabbit
Clone HFG-8
Clonality
  • Monoclonal
Isotype
  • Rabbit IgG
Reactivity
  • Human
  • Mouse
  • Rat
Applications
  • Western Blot
  • Flow Cytometry
Immunogen A synthesized peptide derived from human HDAC7
Molecular weight 102.9 kDa
Purification Affinity-chromatography
Reconstitution Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Cellular localization Nucleus. Cytoplasm. In the nucleus, it associates with distinct subnuclear dot-like structures. Shuttles between the nucleus and the cytoplasm. Treatment with EDN1 results in shuttling from the nucleus to the perinuclear region. The export to cytoplasm depends on the interaction with the 14-3-3 protein YWHAE and is due to its phosphorylation.
Concentration 0.5mg/ml
Form Liquid
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) M01913
Main SKU BHA21008146
Rabbit Monoclonal

Product Overview

Clone HFG-8 is a rabbit monoclonal antibody that recognizes histone deacetylase 7 (gene HDAC7) in human, mouse and rat samples and is validated for Western blot and flow cytometry. The predicted molecular weight is 102.9 kDa, and the supplier reports an observed band at about 124 kDa.

Target Histone deacetylase 7 (gene HDAC7; UniProt Q8WUI4, human)
Clone HFG-8
Host / Clonality / Isotype Rabbit / Monoclonal / Rabbit IgG
Reactivity Human, Mouse, Rat
Form Liquid
Formulation PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers
Calculated MW 102.9 kDa
Observed MW 124 kDa
Storage Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Validated Applications

Western blot 1:500–1:2000
Flow cytometry 1:20

Immunogen

Synthetic peptide based on the sequence of human HDAC7.

Reactivity Notes

The supplier lists reactivity with human, mouse and rat. UniProt places histone deacetylase 7 in the nucleus and cytoplasm.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: Western blot 1:500–1:2000; Flow cytometry 1:20. Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Human, Mouse, Rat. Other species are not listed by the supplier.
Q.What band size should be expected in Western blot?
A.Reported by the supplier: calculated molecular weight 102.9 kDa; observed band at about 124 kDa. A difference from the calculated size is common and can reflect post-translational modification, processing or isoform differences.
Q.What immunogen was used to raise this antibody?
A.Synthetic peptide based on the sequence of human HDAC7.
Q.How should this antibody be stored?
A.Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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