| Field | Specification |
|---|---|
| Target | |
| Alternative names | Guanosine-3',5'-bis; diphosphate; 3'-pyrophosphohydrolase MESH1; HD domain-containing protein 3; Metazoan SpoT homolog 1; MESH1; Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase; HDDC3 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human HDDC3 recombinant protein (Position: K25-I179). Human HDDC3 shares 95.5% amino acid (aa) sequence identity with mouse HDDC3. |
| Purification | |
| Reconstitution | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase MESH1 (gene HDDC3) in human and mouse samples and is validated for Western blot and ELISA.
MESH1 (HDDC3) is the metazoan homolog of bacterial SpoT and hydrolyzes the alarmone (p)ppGpp, a signal of the bacterial stringent response. In human cells, MESH1 also has NADPH phosphatase activity and has been linked to ferroptosis. It is studied in stress responses and redox metabolism.
| Target | Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase MESH1 (gene HDDC3; UniProt Q8N4P3, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Immunogen
Recombinant human HDDC3 fragment (Lys25–Ile179), expressed in E. coli. Sequence identity with the mouse ortholog: 95.5%.
Reactivity Notes
The supplier lists reactivity with human and mouse.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
