Anti-HE4/Wfdc2 Antibody

SKU:BHA21002159
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Suppliers
Boster Bio
Boster Bio
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Overview
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Anti-Wfdc2 antibody (Rabbit polyclonal, Rabbit IgG). Validated for ELISA. Reactivity: Rat. Recognizes WAP four-disulfide core domain protein 2. Supplied in lyophilized form, ready for use in standard immunodetection workflows.
Target Wfdc2
Host Rabbit
Reactivity Rat
Isotype Rabbit IgG
Application(s) WB, IHC, Flow Cytometry, ELISA
Options selector
Catalog no. Size Conjugation
A02685-5 100 ug/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • 100 ug/vial / Carrier Free, 100 ug/vial / Unconjugated: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.; Form: Lyophilized; Applications: ELISA; Application details: ELISA Capture (Matched Antibody Pair), 2-8μg/ml; Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
    • 100 ug/vial / APC, 100 ug/vial / Biotin, 100 ug/vial / Cy3, 100 ug/vial / FITC, 100 ug/vial / Fluoro488, 100 ug/vial / Fluoro550, 100 ug/vial / Fluoro594, 100 ug/vial / Fluoro647, 100 ug/vial / PE: Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.; Form: Liquid; Applications: Flow Cytometry, WB,IHC,ELISA; Application details: Flow Cytometry, 1-3μg/1x106 cells; Western blot, 0.25-0.5μg/ml; Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml; ELISA, 0.1-0.5μg/ml; Storage: At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing., At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
    • 100 ug/vial / HRP: Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4.; Form: Liquid; Applications: WB,IHC,ELISA; Application details: Western blot, 0.25-0.5μg/ml; Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml; ELISA, 0.1-0.5μg/ml; Storage: At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing.
  • Lead time: varies by selected option; please contact us for current fulfillment timing.
  • Storage: varies by selected option; see option details above.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible; avoid repeated freeze-thaw cycles.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target Wfdc2
Alternative names WAP four-disulfide core domain protein 2; Epididymal secretory protein 4; RE4; Wfdc2;
UniProt # Q8CHN3
Host Rabbit
Clonality
  • Polyclonal
Isotype
  • Rabbit IgG
Reactivity
  • Rat
Applications
  • ELISA
Immunogen E. coli-derived rat HE4 recombinant protein (Position: E31-F168).
Molecular weight 17.4 kDa
Purification Immunogen affinity purified.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Cellular localization Secreted .
Concentration Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form Lyophilized
Storage Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) A02685-5
Main SKU BHA21002159
Rabbit Polyclonal

Product Overview

This rabbit polyclonal antibody targets WAP four-disulfide core domain protein 2 (gene Wfdc2) in rat samples and is validated for ELISA. The predicted molecular weight is 17.4 kDa.

WFDC2, better known as HE4 (human epididymis protein 4), is a small secreted protein carrying WAP four-disulfide core domains of the type found in protease inhibitors. It is expressed in epithelia of the reproductive tract and airways, animal studies show it is needed for normal lung and airway epithelial development, and serum HE4 is widely measured in ovarian cancer research.

Target WAP four-disulfide core domain protein 2 (gene Wfdc2; UniProt Q8CHN3, rat)
Host / Clonality / Isotype Rabbit / Polyclonal / Rabbit IgG
Reactivity Rat
Form Lyophilized
Formulation Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3
Calculated MW 17.4 kDa
Storage As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles.

Validated Applications

ELISA (capture antibody) 2–8 µg/mL

Immunogen

Recombinant rat HE4 fragment (Glu31–Phe168), expressed in E. coli.

Reactivity Notes

The supplier lists reactivity with rat. UniProt describes WAP four-disulfide core domain protein 2 as a secreted protein.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: ELISA (capture antibody) 2–8 µg/mL. Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Rat. Other species are not listed by the supplier.
Q.What immunogen was used to raise this antibody?
A.Recombinant rat HE4 fragment (Glu31–Phe168), expressed in E. coli.
Q.How should this antibody be stored?
A.As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

Try Celltrypse Free – Request Your Sample Today