| Field | Specification |
|---|---|
| Target | |
| Alternative names | E3 ubiquitin-protein ligase HECTD3; HECT domain-containing protein 3; HECT-type E3 ubiquitin transferase HECTD3; HECTD3 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human HECTD3, identical to the related mouse and rat sequences. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes E3 ubiquitin-protein ligase HECTD3 in human, mouse and rat samples and is validated for flow cytometry, IHC, ICC/IF and Western blot. The predicted molecular weight is 97.1 kDa, and the supplier reports an observed band at about 97 kDa.
HECTD3 is a HECT-type E3 ubiquitin ligase that transfers ubiquitin directly to its substrates. It targets the actin-binding protein TRIOBP for proteasomal degradation, which supports cell cycle progression, and it also ubiquitinates syntaxin 8. HECTD3 is studied in protein degradation, cell survival and cancer.
| Target | E3 ubiquitin-protein ligase HECTD3 (gene HECTD3; UniProt Q5T447, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 97.1 kDa |
| Observed MW | 97 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|---|
| Flow cytometry | Not specified by the supplier |
| Immunohistochemistry | Not specified by the supplier |
| Immunocytochemistry / Immunofluorescence | Not specified by the supplier |
Samples with a confirmed band (WB): human Caco-2 cells, rat brain tissue, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): human lung cancer tissue, mouse gaster tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Samples with a confirmed signal (flow cytometry): A549 cells.
Immunogen
Synthetic peptide from the C-terminal region of human HECTD3. The peptide sequence is conserved in mouse and rat.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places E3 ubiquitin-protein ligase HECTD3 in the cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Jinjin Yuan et al. (2022) HECTD3 enhances cell radiation resistance and migration by regulating LKB1 mediated ZEB1 in glioma. European Journal of Neuroscience. 10.1111/ejn.15748
