| Field | Specification |
|---|---|
| Target | |
| Alternative names | ATP-dependent RNA helicase with zinc finger domain; Down-regulated in human cancers protein; HELZ; DRHC; KIAA0054 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human HELZ recombinant protein (Position: R81-Q743). Human HELZ shares 95.6% amino acid (aa) sequence identity with mouse HELZ. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects ATP-dependent RNA helicase with zinc finger domain (gene HELZ) in human samples and is validated for Western blot, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 219.0 kDa, and the supplier reports an observed band at about 200 kDa.
HELZ is a large RNA helicase with a zinc finger domain that associates with the CCR4–NOT deadenylase complex. It promotes deadenylation, decapping and decay of target mRNAs and can also repress translation. HELZ is studied in post-transcriptional gene regulation and cell proliferation.
| Target | ATP-dependent RNA helicase with zinc finger domain (gene HELZ; UniProt P42694, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 219.0 kDa |
| Observed MW | 200 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HeLa cells, human MCF-7 cells, human HEL cells, human K562 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): A549 cells.
Samples with a confirmed signal (flow cytometry): HeLa cells, MCF-7 cells.
Immunogen
Recombinant human HELZ fragment (Arg81–Gln743), expressed in E. coli. Sequence identity with the mouse ortholog: 95.6%.
Reactivity Notes
The supplier lists reactivity with human. UniProt places ATP-dependent RNA helicase with zinc finger domain in the nucleus and cytoplasm. Tissue expression noted by UniProt (human): Expressed predominantly in thymus and brain. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
