| Field | Specification |
|---|---|
| Target | |
| Alternative names | Heme oxygenase 1;HO-1;1.14.99.3;HMOX1;HO, HO1; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human HMOX1 recombinant protein (Position: M1-M288). Human HMOX1 shares 82% and 80% amino acid (aa) sequences identity with mouse and rat HMOX1, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects heme oxygenase 1 (gene HMOX1) in human samples and is validated for IHC and Western blot. The predicted molecular weight is 32.8 kDa, and the supplier reports an observed band at about 33 kDa.
Heme oxygenase 1 (HO-1, HMOX1, also called HSP32) breaks down heme into biliverdin, ferrous iron and carbon monoxide. It is strongly induced by oxidative stress, heme and inflammation, and its products have antioxidant and anti-inflammatory effects, so HO-1 is a widely used marker of cellular stress responses and cytoprotection.
| Target | Heme oxygenase 1 (gene HMOX1; UniProt P09601, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 32.8 kDa |
| Observed MW | 33 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
Samples with a confirmed band (WB): human A549 cells, human HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human tonsil tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human HMOX1 fragment (Met1–Met288), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 82% and 80%, respectively.
Reactivity Notes
The supplier lists reactivity with human. UniProt places heme oxygenase 1 in the endoplasmic reticulum membrane. Tissue expression noted by UniProt (human): Expressed at higher levels in renal cancer tissue than in normal tissue (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Guanyu Yang et al. (2021) Fucoxanthin regulates Nrf2 signaling to decrease oxidative stress and improves renal fibrosis depending on Sirt1 in HG-induced GMCs and STZ-induced diabetic rats. European Journal of Pharmacology. 10.1016/j.ejphar.2021.174629
Jun Yao et al. (2020) Nifedipine inhibits oxidative stress and ameliorates osteoarthritis by activating the nuclear factor erythroid-2-related factor 2 pathway. Life Sciences. 10.1016/j.lfs.2020.117292
Shuangchan Wu et al. (2016) Myricetin ameliorates brain injury and neurological deficits via Nrf2 activation after experimental stroke in middle-aged rats. Food & Function. 10.1039/C6FO00419A
Yiming Zhou et al. (2019) Structural and antioxidant analysis of Tartary buckwheat (Fagopyrum tartaricum Gaertn.) 13S globulin. Journal of the Science of Food and Agriculture. 10.1002/jsfa.10133
Han Hedan et al. (2019) Grape Seed Procyanidins Attenuates Cisplatin-induced Human Embryonic Renal Cell Cytotoxicity by Modulating Heme Oxygenase-1 in Vitro. Cell Biochemistry and Biophysics. 10.1007/s12013-019-00890-5
Jiang Ke et al. (2009) Heme oxygenase-1 expression in rats with acute lung rejection and implication. Journal of Huazhong University of Science and Technology-Medical Sciences. 10.1007/s11596-009-0118-0
