| Field | Specification |
|---|---|
| Target | |
| Alternative names | Heme oxygenase 1; HO-1; HSP32; Hmox1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived rat Heme Oxygenase 1/Hmox1 recombinant protein (Position: E2-T261). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes heme oxygenase 1 (gene Hmox1) in rat samples and is validated for Western blot and ELISA. The predicted molecular weight is 33.0 kDa, and the supplier reports an observed band at about 33 kDa.
Heme oxygenase 1 (HO-1, HMOX1, also called HSP32) breaks down heme into biliverdin, ferrous iron and carbon monoxide. It is strongly induced by oxidative stress, heme and inflammation, and its products have antioxidant and anti-inflammatory effects, so HO-1 is a widely used marker of cellular stress responses and cytoprotection.
| Target | Heme oxygenase 1 (gene Hmox1; UniProt P06762, rat) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 33.0 kDa |
| Observed MW | 33 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL/ml (Rat) |
|---|---|
| ELISA | 0.1–0.5 µg/mL/ml (Rat) |
Samples with a confirmed band (WB): rat liver tissue, rat RH35 cells, rat PC-12 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant rat Heme Oxygenase 1/Hmox1 fragment (Glu2–Thr261), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with rat. UniProt places heme oxygenase 1 in the endoplasmic reticulum membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Zhang Xin et al. (2024) Daphnetin protects neurons in an Alzheimer disease mouse model and normal rat neurons by inhibiting BACE1 activity and activating the Nrf2/HO-1 pathway. Journal of Neuropathology and Experimental Neurology. 10.1093/jnen/nlae043
Xuanshuo Liu et al. (2020) Shunaoxin pills improve the antihypertensive effect of nifedipine and alleviate its renal lipotoxicity in spontaneous hypertension rats. Environmental Toxicology. 10.1002/tox.23044
Xiaoyu Zhu et al. (2018) Astaxanthin Promotes Nrf2/ARE Signaling to Alleviate Renal Fibronectin and Collagen IV Accumulation in Diabetic Rats. Journal of Diabetes Research. 10.1155/2018/6730315
Sun Shuping et al. (2019) Water-separated part of Chloranthus serratus alleviates lipopolysaccharide- induced RAW264.7 cell injury mainly by regulating the MAPK and Nrf2/HO-1 inflammatory pathways. BMC Complementary and Alternative Medicine. 10.1186/s12906-019-2755-6
Yeying Wang et al. (2018) Tempol relieves lung injury in a rat model of chronic intermittent hypoxia via suppression of inflammation and oxidative stress. Iranian Journal of Basic Medical Sciences. 10.22038/ijbms.2018.31716.7714
