| Field | Specification |
|---|---|
| Target | |
| Alternative names | Homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 1 protein; Methyl methanesulfonate (MMF)-inducible fragment protein 1; HERPUD1; HERP; KIAA0025; MIF1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human HERP/HERPUD1 recombinant protein (Position: E6-Q361). Human HERPUD1 shares 88.7% amino acid (aa) sequence identity with mouse HERPUD1. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 1 protein (gene HERPUD1) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and ELISA. The predicted molecular weight is 43.7 kDa, and the supplier reports an observed band at about 56 kDa.
HERPUD1 (Herp) is an endoplasmic reticulum membrane protein with a ubiquitin-like domain that is strongly induced by ER stress. It supports ER-associated degradation (ERAD) of misfolded proteins by helping assemble the HRD1 ubiquitin ligase machinery and interacting with ubiquilins. HERPUD1 is studied in the unfolded protein response and ER protein quality control.
| Target | Homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 1 protein (gene HERPUD1; UniProt Q15011, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 43.7 kDa |
| Observed MW | 56 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human PC-3 cells, human HeLa cells, human HepG2 cells, human LNCAP cells, rat liver tissue, mouse liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human appendiceal carcinoid tissue, human breast cancer tissue, human cervix squamous cells carcinoma tissue, human ovarian cancer tissue, human rectum adenocarcinoma tissue, human testicular seminoma tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): A549 cells.
Samples with confirmed staining (ICC/IF): human appendix tissue, human ovarian tissue.
Immunogen
Recombinant human HERP/HERPUD1 fragment (Glu6–Gln361), expressed in E. coli. Sequence identity with the mouse ortholog: 88.7%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 1 protein in the endoplasmic reticulum membrane. Tissue expression noted by UniProt (human): Widely expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
