| Field | Specification |
|---|---|
| Target | |
| Alternative names | Transcription factor HES-1;Class B basic helix-loop-helix protein 39;bHLHb39;Hairy and enhancer of split 1;Hairy homolog;Hairy-like protein;hHL;HES1;BHLHB39, HL, HRY; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Hes1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone FBB-8 is a rabbit monoclonal antibody that recognizes transcription factor HES-1 (gene HES1) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 29.5 kDa, and the supplier reports an observed band at about 30 kDa.
HES1 is a basic helix-loop-helix transcriptional repressor and a primary target of Notch signaling. It binds N-box sites and represses proneural and differentiation genes, keeping progenitor and stem cells undifferentiated, and its expression oscillates in many cells. HES1 is widely studied in neural development, stem cell maintenance and cancer.
| Target | Transcription factor HES-1 (gene HES1; UniProt Q14469, human) |
|---|---|
| Clone | FBB-8 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 29.5 kDa |
| Observed MW | 30 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): human U-87MG cells, human U251 cells, human 293T cells, rat brain tissue, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Samples with confirmed staining (IHC): human liver tissue, human breast cancer tissue, rat brain tissue, rat cerebellum tissue, rat kidney tissue, human thyriod cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): rat kidney tissue, human breast cancer tissue, mouse brain tissue, mouse cerebellum tissue, human pancreas cancer tissue.
Immunogen
Synthetic peptide based on the sequence of human hes1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places transcription factor HES-1 in the nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Jinsong Su et al. (2023) Tibetan medicine Qi-Sai-Er-Sang-Dang-Song Decoction inhibits TNF-α-induced rheumatoid arthritis in human fibroblast-like synoviocytes via regulating NOTCH1/NF-κB/NLRP3 pathway. Journal of Ethnopharmacology. 10.1016/j.jep.2023.116402
Xudong Wang et al. (2026) Therapeutic potential of recombinant human collagen XVII in blue light-induced skin photoaging: preserving epidermal-dermal structural integrity and functional homeostasis. Frontiers in Bioengineering and Biotechnology. 10.3389/fbioe.2026.1806274
Jianfei Shi et al. (2020) Matrine promotes hepatic oval cells differentiation into hepatocytes and alleviates liver injury by suppression of Notch signalling pathway. Life Sciences. 10.1016/j.lfs.2020.118354
Guangmao Zhou et al. (2026) Single-cell transcriptomics reveals CCL2-mediated macrophage-endothelial cell interactions drive apoptosis in varicose veins. Gene. 10.1016/j.gene.2026.150279
Shu Yin et al. (2025) Demethylated miR-184 regulates EPB41L5 and downregulates Notch signaling to inhibit metastasis in colorectal cancer. Journal of Molecular Histology. 10.1007/s10735-025-10434-2
Junhong Guan et al. (2017) Osthole prevents cerebral ischemia–reperfusion injury via the Notch signaling pathway. Biochemistry and Cell Biology. 10.1139/bcb-2016-0233
Linlin Lu et al. (2019) Salidroside suppresses the metastasis of hepatocellular carcinoma cells by inhibiting the activation of the Notch1 signaling pathway. Molecular Medicine Reports. 10.3892/mmr.2019.10115
Jianfei Shi et al. (2019) Matrine prevents the early development of hepatocellular carcinoma like lesions in rat liver. Experimental and Therapeutic Medicine. 10.3892/etm.2019.7875
