| Field | Specification |
|---|---|
| Target | |
| Alternative names | Transcription factor HES-5; Class B basic helix-loop-helix protein 38; bHLHb38; Hairy and enhancer of split 5; HES5 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human HES5 Transcriptional repressor of genes that require a bHLH protein for their transcription. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AEGI-8 is a rabbit monoclonal antibody that recognizes transcription factor HES-5 (gene HES5) in human, mouse and rat samples and is validated for Western blot, IHC and immunoprecipitation. The predicted molecular weight is 18.2 kDa, and the supplier reports an observed band at about 18 kDa.
HES5 is a basic helix-loop-helix transcriptional repressor induced by Notch signaling, mainly in the developing nervous system. It suppresses proneural gene activity and so maintains neural stem and progenitor cells, working partly redundantly with HES1. HES5 is studied in neurogenesis and gliogenesis.
| Target | Transcription factor HES-5 (gene HES5; UniProt Q5TA89, human) |
|---|---|
| Clone | AEGI-8 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 18.2 kDa |
| Observed MW | 18 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): human U251 cells, rat spleen tissue, human RAW264.7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human HES5.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places transcription factor HES-5 in the nucleus. Tissue expression noted by UniProt (human): Expressed in fetal heart and brain tumors. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
