| Field | Specification |
|---|---|
| Target | |
| Alternative names | Hexokinase-2;2.7.1.1;Hexokinase type II;HK II;Muscle form hexokinase;HK2; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human Hexokinase II, different from the related mouse and rat sequences by four amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets hexokinase-2 (gene HK2) in human, mouse and rat samples and is validated for flow cytometry, IHC and Western blot. The predicted molecular weight is 102.4 kDa, and the supplier reports an observed band at about 102 kDa.
Hexokinase 2 (HK2) phosphorylates glucose to glucose 6-phosphate, the first committed step of glycolysis, and is the main hexokinase in insulin-sensitive tissues such as muscle and fat. It binds the outer mitochondrial membrane, where it helps protect cells from apoptosis, and its high expression in many tumors makes it a focus of cancer metabolism research.
| Target | Hexokinase-2 (gene HK2; UniProt P52789, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 102.4 kDa |
| Observed MW | 102 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Mouse, Rat) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human HeLa cells, human MCF-7 cells, human HepG2 cells, human HeLa- WT cells, human Hela-HK2 KO cells, rat skeletal muscle tissue, rat heart tissue, mouse skeletal muscle tissue, mouse heart tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 8% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human breast cancer tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with a confirmed signal (flow cytometry): HepG2 cells.
Immunogen
Synthetic peptide from the middle region of human hexokinase II. It differs from the mouse and rat sequences at four residues.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places hexokinase-2 in the mitochondrion outer membrane and cytoplasm. Tissue expression noted by UniProt (human): Predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yu Meng et al. (2025) HCMV immediate-early protein IE2 induces neurotoxicity and hearing loss by disrupting TSC2-mTOR signaling and metabolic homeostasis. Journal of Neuroinflammation. 10.1186/s12974-025-03646-6
Okuno Keisuke et al. (2023) Infused juice concentrate of Japanese plum Prunus mume attenuates inflammatory vascular remodeling in a mouse model of hypertension induced by angiotensin II. Hypertension Research. 10.1038/s41440-023-01332-9
Keiichi Torimoto et al. (2022) Glucose consumption of vascular cell types in culture: toward optimization of experimental conditions. American Journal of Physiology-Cell Physiology. 10.1152/ajpcell.00257.2021
June Bai et al. (2025) Mitochondrial Genome-Encoded lncND5 Regulates Mitophagy in Hypoxic Pulmonary Artery Smooth Muscle Cell. FASEB Journal. 10.1096/fj.202500389R
Long Niya et al. (2020) CircPOSTN/miR-361-5p/TPX2 axis regulates cell growth, apoptosis and aerobic glycolysis in glioma cells. Cancer Cell International. 10.1186/s12935-020-01454-x
Cheng Sha et al. (2026) The fluoroquinoline compound exerts anti-erythroleukemic effects by dual-targeting GLUT1 and the PI3K/AKT signaling pathway. Scientific Reports. 10.1038/s41598-026-45597-9
