| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Hereditary hemochromatosis protein;HLA-H;HFE;HLAH; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human HFE recombinant protein (Position: Q82-R199). Human HFE shares 72.2% and 74.6% amino acid (aa) sequence identity with mouse and rat HFE, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of HFE (Hereditary hemochromatosis protein) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-HFE Antibody Picoband® catalog # A00506-1. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E.coli-derived human HFE recombinant protein (Position: Q82-R199). Human HFE shares 72.2% and 74.6% amino acid (aa) sequence identity with mouse and rat HFE, respectively. (reported region: Q82-R199).
- Molecular weight context: reported MW: 40 kDa; calculated MW: 40108 MW
- Reactivity: Human
- Applications: WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Hereditary hemochromatosis protein; Hereditary hemochromatosis protein. Human hemochromatosis protein also known as the HFE protein is a protein which in humans is encoded by the HFE gene. The HFE gene is located on short arm of chromosome 6 at location 6p21.3. The protein encoded by this gene is a membrane protein that is similar to MHC class I-type proteins and associates with beta2-microglobulin (beta2M). It is thought that this protein functions to regulate iron absorption by regulating the interaction of the transferrin receptor with transferrin. The iron storage disorder, hereditary haemochromatosis, is a recessive genetic disorder that results from defects in this gene. Functional note: Binds to transferrin receptor (TFR) and reduces its affinity for iron-loaded transferrin. . Reported localization: Cell membrane ; Single-pass type I membrane protein . Expression/tissue context: Expressed in all tissues tested except brain.
Research relevance and current trends
- Cofactors: Researchers commonly examine how HFE (Hereditary hemochromatosis protein) relates to this theme using model systems and orthogonal readouts.
- Vitamins/Minerals: Researchers commonly examine how HFE (Hereditary hemochromatosis protein) relates to this theme using model systems and orthogonal readouts.
- Hematopoietic Progenitors: Researchers commonly examine how HFE (Hereditary hemochromatosis protein) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative HFE (Hereditary hemochromatosis protein) levels across conditions; band patterns may reflect isoforms and processing.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
