| Field | Specification |
|---|---|
| Target | |
| Alternative names | Homogentisate 1,2-dioxygenase; Homogentisate oxygenase; Homogentisic acid oxidase; Homogentisicase; HGD; HGO |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human HGD |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 27H27 is a rabbit monoclonal antibody that recognizes homogentisate 1,2-dioxygenase (gene HGD) in human samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 50.0 kDa, and the supplier reports an observed band at about 50 kDa.
Homogentisate 1,2-dioxygenase (HGD) is an iron-dependent enzyme of the tyrosine and phenylalanine breakdown pathway that opens the aromatic ring of homogentisate to give maleylacetoacetate. It is expressed mainly in liver and kidney, and loss-of-function mutations in HGD cause alkaptonuria, which makes the enzyme a reference point in studies of inherited amino acid metabolism.
| Target | Homogentisate 1,2-dioxygenase (gene HGD; UniProt Q93099, human) |
|---|---|
| Clone | 27H27 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 50.0 kDa |
| Observed MW | 50 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): HepG2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human HGD.
Reactivity Notes
The supplier lists reactivity with human. Tissue expression noted by UniProt (human): Highest expression in the prostate, small intestine, colon, kidney and liver. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
