| Field | Specification |
|---|---|
| Target | |
| Alternative names | Hypoxia-inducible factor 1-alpha; HIF-1-alpha; HIF1-alpha; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78; bHLHe78; PAS domain-containing protein 8; HIF1A; BHLHE78, MOP1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human HIF-1 alpha/HIF1A recombinant protein (Position: Q603-Q814). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets hypoxia-inducible factor 1-alpha (gene HIF1A) in human, mouse and rat samples and is validated for Western blot, IHC, flow cytometry and ELISA. The predicted molecular weight is 92.7 kDa, and the supplier reports an observed band at about 93 kDa.
HIF-1α (HIF1A) is the oxygen-regulated subunit of hypoxia-inducible factor 1. In normal oxygen it is hydroxylated and rapidly degraded, but under hypoxia it accumulates, pairs with ARNT (HIF-1β) and switches on genes for erythropoietin, VEGF, glucose uptake and glycolysis. It is a central marker in studies of hypoxia, angiogenesis, tumor metabolism and ischemia.
| Target | Hypoxia-inducible factor 1-alpha (gene HIF1A; UniProt Q16665, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg NaN3 |
| Calculated MW | 92.7 kDa |
| Observed MW | 93 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Mouse, Rat) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human A431 cells, human HepG2 cells, human U2OS cells, rat PC-12 cells, mouse thymus tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human mammary cancer tissue, human rectal cancer tissue, mouse intestine tissue, rat intestine tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with a confirmed signal (flow cytometry): A431 cells.
Immunogen
Recombinant human HIF-1 alpha/HIF1A fragment (Gln603–Gln814), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places hypoxia-inducible factor 1-alpha in the cytoplasm, nucleus and nucleus speckle. Tissue expression noted by UniProt (human): Expressed in most tissues with highest levels in kidney and heart. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yi Lingxian et al. (2021) Intraperitoneal injection of Desferal® alleviated the age-related bone loss and senescence of bone marrow stromal cells in rats. Stem Cell Research & Therapy. 10.1186/s13287-020-02112-9
Xiang Zeng et al. (2011) Bone Marrow Mesenchymal Stem Cells in a Three-Dimensional Gelatin Sponge Scaffold Attenuate Inflammation, Promote Angiogenesis, and Reduce Cavity Formation in Experimental Spinal Cord Injury. Cell Transplantation. 10.3727/096368911X566181
Zhang Runfang et al. (2021) Curcumin Derivative Cur20 Attenuated Cerebral Ischemic Injury by Antioxidant Effect and HIF-1α/VEGF/TFEB-Activated Angiogenesis. Frontiers in Pharmacology. 10.3389/fphar.2021.648107
Hongchi Jiang et al. (2007) Antiangiogenic therapy enhances the efficacy of transcatheter arterial embolization for hepatocellular carcinomas. International Journal of Cancer. 10.1002/ijc.22655
Wan Jun et al. (2016) Hyperthermia induced HIF-1a expression of lung cancer through AKT and ERK signaling pathways. Journal of Experimental & Clinical Cancer Research. 10.1186/s13046-016-0399-7
Jiali Zhao et al. (2022) Combined Treatment of Tanshinone I and Epirubicin Revealed Enhanced Inhibition of Hepatocellular Carcinoma by Targeting PI3K/AKT/HIF-1α. Drug Design Development and Therapy. 10.2147/dddt.s360691
Xue-Pu Sun et al. (2013) Up-regulation of survivin by AKT and hypoxia-inducible factor 1α contributes to cisplatin resistance in gastric cancer. FEBS Journal. 10.1111/febs.12577
Liu Pian et al. (2011) Combinational Therapy of Interferon-α and Chemotherapy Normalizes Tumor Vasculature by Regulating Pericytes Including the Novel Marker RGS5 in Melanoma. Journal of Immunotherapy. 10.1097/CJI.0b013e318213cd12
Fengjun Liu et al. (2008) Antisense hypoxia-inducible factor 1α gene therapy enhances the therapeutic efficacy of doxorubicin to combat hepatocellular carcinoma. Cancer Science. 10.1111/j.1349-7006.2008.00905.x
Shiguang Huang et al. (2011) The Role of Psychologic Stress-Induced Hypoxia-Inducible Factor-1α in Rat Experimental Periodontitis. Journal of Periodontology. 10.1902/jop.2010.100610
