| Field | Specification |
|---|---|
| Target | |
| Alternative names | Hypoxia-inducible factor 1-alpha; HIF-1-alpha; HIF1-alpha; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78; bHLHe78; Member of PAS protein 1; PAS domain-containing protein 8; HIF1A; MOP1; PASD8 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human HIF-1-alpha |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 31H88 is a rabbit monoclonal antibody that recognizes hypoxia-inducible factor 1-alpha (gene HIF1A) in human, mouse and rat samples and is validated for Western blot and ICC/IF. The predicted molecular weight is 92.7 kDa, and the supplier reports an observed band at about 110 kDa.
HIF-1α (HIF1A) is the oxygen-regulated subunit of hypoxia-inducible factor 1. In normal oxygen it is hydroxylated and rapidly degraded, but under hypoxia it accumulates, pairs with ARNT (HIF-1β) and switches on genes for erythropoietin, VEGF, glucose uptake and glycolysis. It is a central marker in studies of hypoxia, angiogenesis, tumor metabolism and ischemia.
| Target | Hypoxia-inducible factor 1-alpha (gene HIF1A; UniProt Q16665, human) |
|---|---|
| Clone | 31H88 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 92.7 kDa |
| Observed MW | 110 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human HIF-1-alpha.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places hypoxia-inducible factor 1-alpha in the cytoplasm, nucleus and nucleus speckle. Tissue expression noted by UniProt (human): Expressed in most tissues with highest levels in kidney and heart.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Hu Qiang et al. (2026) Divergent mechanisms, unified therapy: saracatinib targets Src/HIF signaling in endothelial and microglial cells to Treat retinopathy. Biochemical Pharmacology. 10.1016/j.bcp.2026.118028
Haibo Li et al. (2025) Regulation of endothelin receptor expression by microRNAs in diabetes related erectile dysfunction. Biochemical Pharmacology. 10.1016/j.bcp.2025.117397
Ying Shen et al. (2025) Synergistic effects of diammonium glycyrrhizinate and Vitamin D3 against EMT in pulmonary fibrosis through modulating the crosstalk of STAT3/HSP90AA1 and HIF-1α pathway. Journal of Ethnopharmacology. 10.1016/j.jep.2025.120649
Yue Shi et al. (2025) 4EBP1 phosphorylation mediates TGF-β1–induced hepatic fibrosis and upregulates HIF-1α expression. iScience. 10.1016/j.isci.2025.113412
Zhang Yan et al. (2024) Prognostic implications of TOR1B expression across cancer types: a focus on basal-like breast cancer and cellular adaptations to hypoxia. Journal of Cancer Research and Clinical Oncology. 10.1007/s00432-024-05794-3
Jian Wu et al. (2025) Hypoxia inducible factor-1alpha expression correlates with inflammatory injury of blood-brain barrier which influences perihaematomal edema after intracerebral hemorrhage. Journal of Stroke & Cerebrovascular Diseases. 10.1016/j.jstrokecerebrovasdis.2025.108269
