| Field | Specification |
|---|---|
| Target | |
| Alternative names | Endothelial PAS domain-containing protein 1; EPAS-1; Basic-helix-loop-helix-PAS protein MOP2; Class E basic helix-loop-helix protein 73; bHLHe73; HIF-1-alpha-like factor; HLF; Hypoxia-inducible factor 2-alpha; HIF-2-alpha; HIF2-alpha; Member of PAS protein 2; PAS domain-containing protein 2; EPAS1; HIF2A; MOP2; PASD2 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human HIF-2-alpha/EPAS1, which shares 94.4% amino acid (aa) sequence identity with both mouse and rat HIF-2-alpha/EPAS1. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets endothelial PAS domain-containing protein 1 (gene EPAS1) in human, mouse and rat samples and is validated for Western blot and flow cytometry. The predicted molecular weight is 96.5 kDa, and the supplier reports an observed band at about 96 kDa.
HIF-2α (EPAS1) is an oxygen-regulated transcription factor. Under low oxygen it escapes VHL-mediated degradation, pairs with ARNT (HIF-1β) and activates hypoxia-responsive genes such as VEGF and erythropoietin. HIF-2α is studied in vascular biology, erythropoiesis, high-altitude adaptation and clear cell renal carcinoma.
| Target | Endothelial PAS domain-containing protein 1 (gene EPAS1; UniProt Q99814, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg NaN3 |
| Calculated MW | 96.5 kDa |
| Observed MW | 96 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): rat lung tissue, rat PC-12 cells, mouse lung tissue, mouse SP2/0 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): SiHa cells.
Immunogen
Synthetic peptide from the C-terminal region of human HIF-2-alpha/EPAS1. Sequence identity with the mouse and rat orthologs: 94.4%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places endothelial PAS domain-containing protein 1 in the nucleus and nucleus speckle. Tissue expression noted by UniProt (human): Expressed in most tissues, with highest levels in placenta, lung and heart. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
