| Field | Specification |
|---|---|
| Target | |
| Alternative names | Hypoxia-inducible factor 3-alpha; HIF-3-alpha; HIF3-alpha; Basic-helix-loop-helix-PAS protein MOP7; Class E basic helix-loop-helix protein 17; bHLHe17; HIF3-alpha-1; Inhibitory PAS domain protein; IPAS; Member of PAS protein 7; PAS domain-containing protein 7; HIF3A; BHLHE17, MOP7, PASD7 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human HIF3/HIF3A recombinant protein (Position: Q29-Q480). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects hypoxia-inducible factor 3-alpha (gene HIF3A) in human, mouse and rat samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 72.4 kDa, and the supplier reports an observed band at about 72 kDa.
HIF-3α (HIF3A) is the third oxygen-regulated α subunit of the hypoxia-inducible factor family. It can partner with ARNT, and several of its splice forms act as dominant-negative regulators that reduce HIF-1α and HIF-2α target gene activation, so it is studied as a modulator of the hypoxic response.
| Target | Hypoxia-inducible factor 3-alpha (gene HIF3A; UniProt Q9Y2N7, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 72.4 kDa |
| Observed MW | 72 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HeLa cells, human A431 cells, human Caco-2 cells, human U-87MG cells, human U2OS cells, human K562 cells, human PC-3 cells, rat lung tissue, rat skeletal muscle tissue, mouse lung tissue, mouse NIH3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with a confirmed signal (flow cytometry): U87 cells.
Immunogen
Recombinant human HIF3/HIF3A fragment (Gln29–Gln480), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places hypoxia-inducible factor 3-alpha in the nucleus, cytoplasm, nucleus speckle and mitochondrion. Tissue expression noted by UniProt (human): Expressed in vascular cells (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
