| Field | Specification |
|---|---|
| Target | |
| Alternative names | Neuron-specific calcium-binding protein hippocalcin; Calcium-binding protein BDR-2; HPCA; BDR2 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Hippocalcin |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 24H66 is a rabbit monoclonal antibody that recognizes neuron-specific calcium-binding protein hippocalcin (gene HPCA) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 22.4 kDa, and the supplier reports an observed band at about 26 kDa.
Hippocalcin (HPCA) is a neuronal calcium sensor protein that is highly expressed in the hippocampus. When it binds Ca2+ it attaches to membranes through its myristoyl group and modulates channels and signaling enzymes, and it is studied in synaptic plasticity and neuronal calcium signaling. HPCA mutations cause a form of inherited dystonia.
| Target | Neuron-specific calcium-binding protein hippocalcin (gene HPCA; UniProt P84074, human) |
|---|---|
| Clone | 24H66 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 22.4 kDa |
| Observed MW | 26 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): Neuro2a cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human hippocalcin.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places neuron-specific calcium-binding protein hippocalcin in the cytoplasm and membrane. Tissue expression noted by UniProt (human): Brain specific. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
