| Field | Specification |
|---|---|
| Target | |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence of His Tag. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 19b10 is a mouse monoclonal antibody that recognizes the His tag and is validated for Western blot. The predicted molecular weight is 8.4 kDa, and the supplier reports an observed band at about 12 kDa.
| Target | His tag |
|---|---|
| Clone | 19b10 |
| Host / Clonality / Isotype | Mouse / Monoclonal / Mouse IgG2b |
| Reactivity | All species |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 8.4 kDa |
| Observed MW | 12 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|
Conditions in the example images (WB): 5–20% gradient SDS-PAGE, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of his Tag.
Reactivity Notes
This antibody detects the His tag only on proteins that carry it; it does not recognize an endogenous protein.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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Guihua Zhang et al. (2023) Oral Immunization with Attenuated Salmonella Choleraesuis Expressing the FedF Antigens Protects Mice against the Shiga-Toxin-Producing Escherichia coli Challenge. Biomolecules. 10.3390/biom13121726
Cui Jing et al. (2016) Glucosidase II β-subunit, a novel substrate for caspase-3-like activity in rice, plays as a molecular switch between autophagy and programmed cell death. Scientific Reports. 10.1038/srep31764
Quan Li et al. (2017) The non-conserved region of MRP is involved in the virulence of Streptococcus suis serotype 2. Virulence. 10.1080/21505594.2017.1313373
Chen Bin et al. (2024) Surface Display of Duck Hepatitis A Virus Type 1 VP1 Protein on Bacillus subtilis Spores Elicits Specific Systemic and Mucosal Immune Responses on Mice. Probiotics and Antimicrobial Proteins. 10.1007/s12602-024-10323-2
Wang Jicheng et al. (2024) Dual effects of tomato chlorosis virus on its whitefly vector and its host plant to facilitate viral spread. Journal of Pest Science. 10.1007/s10340-024-01814-w
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