| Field | Specification |
|---|---|
| Target | |
| Alternative names | Histone H2B type 1-K;H2B K;HIRA-interacting protein 1;HIST1H2BK;H2BFT, HIRIP1; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Histone H2B |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone HAD-8 is a rabbit monoclonal antibody that recognizes histone H2B type 1-K (gene H2BC12) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 13.9 kDa, and the supplier reports an observed band at about 17 kDa.
Histone H2B type 1-K (H2BC12) is a canonical H2B, one of the four core histones that make up the nucleosome. Nucleosomes package DNA into chromatin and control its access to transcription, replication and repair machinery, with post-translational histone marks adding a further layer of regulation. H2B ubiquitination and acetylation are well-known marks of active transcription.
| Target | Histone H2B type 1-K (gene H2BC12; UniProt O60814, human) |
|---|---|
| Clone | HAD-8 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 13.9 kDa |
| Observed MW | 17 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:1000–1:5000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:30 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): human A431 cells, human SIHA cells, rat brain tissue, rat liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 12% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human lung cancer tissue, rat uterus tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with a confirmed result (IP): K562 cells.
Immunogen
Synthetic peptide based on the sequence of human histone H2B.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places histone H2B type 1-K in the nucleus and chromosome. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Si X. Xu et al. (2022) The binding between NPM and H2B proteins signals for the diabetes-associated centrosome amplification. Cell Biochemistry and Function. 10.1002/cbf.3721
