| Field | Specification |
|---|---|
| Target | |
| Alternative names | HLA class II histocompatibility antigen, DP beta 1 chain;HLA class II histocompatibility antigen, DP (W4) beta chain;MHC class II antigen DPB1;HLA-DPB1;HLA-DP1B; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human HLA-DPB1 recombinant protein (Position: R30-A258). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets HLA class II histocompatibility antigen, DP beta 1 chain (gene HLA-DPB1) in human samples and is validated for IHC and Western blot. The predicted molecular weight is 29.2 kDa, and the supplier reports an observed band at about 29 kDa.
HLA-DPB1 encodes the β chain of HLA-DP, one of the classical MHC class II molecules. Paired with its α chain on antigen-presenting cells, it displays peptides from proteins taken up by endocytosis to CD4+ helper T cells. The gene is highly polymorphic and is typed in transplantation and immunogenetic studies.
| Target | HLA class II histocompatibility antigen, DP beta 1 chain (gene HLA-DPB1; UniProt P04440, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg NaN3; plus stabilizers |
| Calculated MW | 29.2 kDa |
| Observed MW | 29 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
Samples with a confirmed band (WB): human Raji cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human liver cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human HLA-DPB1 fragment (Arg30–Ala258), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places HLA class II histocompatibility antigen, DP beta 1 chain in the cell membrane, endoplasmic reticulum membrane, endosome membrane and lysosome membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
