| Field | Specification |
|---|---|
| Target | |
| Alternative names | HLA class II histocompatibility antigen, DR beta 4 chain; MHC class II antigen DRB4; HLA-DRB4 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from HLA-DRB4 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 26H61 is a rabbit monoclonal antibody that recognizes HLA class II histocompatibility antigen, DR beta 4 chain (gene HLA-DRB4) in human samples and is validated for Western blot, IHC and flow cytometry. The predicted molecular weight is 29.9 kDa, and the supplier reports an observed band at about 30 kDa.
HLA-DRB4 encodes a secondary HLA-DR β chain (the DR53 group) present only on certain HLA haplotypes. It pairs with HLA-DRA to form an additional class II molecule that presents peptides to CD4+ T cells, and it is considered in HLA typing and immunogenetic studies.
| Target | HLA class II histocompatibility antigen, DR beta 4 chain (gene HLA-DRB4; UniProt P13762, human) |
|---|---|
| Clone | 26H61 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 29.9 kDa |
| Observed MW | 30 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): Raji cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of HLA-DRB4.
Reactivity Notes
The supplier lists reactivity with human. UniProt places HLA class II histocompatibility antigen, DR beta 4 chain in the cell membrane, endoplasmic reticulum membrane, endosome membrane and lysosome membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
