| Field | Specification |
|---|---|
| Target | |
| Alternative names | DNA-dependent ATPase/E3 ubiquitin-protein ligase HLTF; DNA-binding protein/plasminogen activator inhibitor 1 regulator; HIP116; Helicase-like transcription factor; HLTF; RING finger protein 80; RING-type E3 ubiquitin transferase HLTF; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 3; SMARCA3; Sucrose nonfermenting protein 2-like 3; HIP116A; RNF80; SNF2L3; ZBU1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human HLTF |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 23H05 is a rabbit monoclonal antibody that recognizes DNA-dependent ATPase/E3 ubiquitin-protein ligase HLTF in human and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 113.9 kDa, and the supplier reports an observed band at about 120 kDa.
HLTF is a SWI/SNF-family DNA-dependent ATPase that also works as a RING E3 ubiquitin ligase. It polyubiquitinates PCNA to support error-free DNA damage tolerance and can remodel stalled replication forks, and it has additional roles in chromatin regulation. Its gene is often silenced by methylation in colorectal and other cancers.
| Target | DNA-dependent ATPase/E3 ubiquitin-protein ligase HLTF (gene HLTF; UniProt Q14527, human) |
|---|---|
| Clone | 23H05 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 113.9 kDa |
| Observed MW | 120 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:1000–1:5000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): K562 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human HLTF.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places DNA-dependent ATPase/E3 ubiquitin-protein ligase HLTF in the nucleus and chromosome. Tissue expression noted by UniProt (human): Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
