| Field | Specification |
|---|---|
| Target | |
| Alternative names | Porphobilinogen deaminase;PBG-D;2.5.1.61;Hydroxymethylbilane synthase;HMBS;Pre-uroporphyrinogen synthase;HMBS;PBGD, UPS; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human HMBS |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone FEG-8 is a rabbit monoclonal antibody that recognizes porphobilinogen deaminase (gene HMBS) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 39.3 kDa, and the supplier reports an observed band at about 42 kDa.
Porphobilinogen deaminase (HMBS, also called hydroxymethylbilane synthase) is the third enzyme of heme biosynthesis. It links four porphobilinogen units into the linear tetrapyrrole hydroxymethylbilane, building the chain on its own dipyrromethane cofactor. HMBS deficiency causes acute intermittent porphyria.
| Target | Porphobilinogen deaminase (gene HMBS; UniProt P08397, human) |
|---|---|
| Clone | FEG-8 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 39.3 kDa |
| Observed MW | 42 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): HepG2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human HMBS.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places porphobilinogen deaminase in the cytoplasm. Tissue expression noted by UniProt (human): Is ubiquitously expressed... The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
