| Field | Specification |
|---|---|
| Target | |
| Alternative names | High mobility group protein B3; High mobility group protein 2a; HMG-2a; High mobility group protein 4; HMG-4; HMGB3; HMG2A; HMG4 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human HMG4 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 28H64 is a rabbit monoclonal antibody that recognizes high mobility group protein B3 (gene HMGB3) in human and mouse samples and is validated for Western blot. The predicted molecular weight is 23.0 kDa, and the supplier reports an observed band at about 23 kDa.
HMGB3 is an HMG-box chromatin protein that binds DNA with a preference for non-standard structures and can bend it. It is expressed mainly in hematopoietic and embryonic tissues, where it helps balance stem cell self-renewal and differentiation, and it is studied in hematopoiesis and cancer.
| Target | High mobility group protein B3 (gene HMGB3; UniProt O15347, human) |
|---|---|
| Clone | 28H64 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 23.0 kDa |
| Observed MW | 23 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human HMG4.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places high mobility group protein B3 in the nucleus, chromosome and cytoplasm. Tissue expression noted by UniProt (human): Expressed predominantly in placenta. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Zhong Xiaodan et al. (2021) HMGB3 is Associated With an Unfavorable Prognosis of Neuroblastoma and Promotes Tumor Progression by Mediating TPX2. Frontiers in Cell and Developmental Biology. 10.3389/fcell.2021.769547
