| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Endothelin B receptor;ET-B;ET-BR;Endothelin receptor non-selective type;EDNRB;ETRB; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Gene ID | |
| Host | |
| Immunogen | A synthesized peptide derived from human HMGA1 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-HMGA1 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 18H23; isotype IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, IHC, ICC, IF, Flow (as provided in the source record). Boster Bio Anti-HMGA1 Rabbit Monoclonal Antibody catalog # M01043. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: HMGA1 (Endothelin B receptor).
- Antibody format: Monoclonal; clone 18H23; isotype IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
HMGA1 (protein: Glycogen synthase kinase-3 beta (gsk3b)) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Non-specific receptor for endothelin 1, 2, and 3. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. . Reported cellular localization context: Cell membrane; Multi-pass membrane protein. Tissue expression notes (as provided): Expressed in placental stem villi vessels, but not in cultured placental villi smooth muscle cells. .
Research relevance and current trends
- Research context keywords from the source record include: G Protein Signaling,Signal Transduction,Signaling Pathway.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.
Workflow ideas (metafield): Validate HMGA1 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect HMGA1 expression by Western blot in cell or tissue lysates, Detect HMGA1 in FFPE tissue sections by immunohistochemistry, Localize HMGA1 by immunofluorescence/immunocytochemistry in cultured cells, Quantify HMGA1-positive cells by flow cytometry in single-cell suspensions
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 17 kDa; calculated MW: 49644 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 17 kDa
- Cellular localization (provided): Cell membrane; Multi-pass membrane protein.
- Tissue details (provided): Expressed in placental stem villi vessels, but not in cultured placental villi smooth muscle cells. .
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
