| Field | Specification |
|---|---|
| Target | |
| Alternative names | High mobility group protein B2; High mobility group protein 2; HMG-2; HMGB2; HMG2 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human HMGB2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ACCF-8 is a rabbit monoclonal antibody that recognizes high mobility group protein B2 (gene HMGB2) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and immunoprecipitation. The predicted molecular weight is 24.0 kDa, and the supplier reports an observed band at about 24 kDa.
HMGB2 is a chromatin-associated HMG-box protein that binds DNA with a preference for bent or unusual structures and increases DNA flexibility, helping transcription factors and the RAG recombinase act. It is highly expressed in proliferating cells, lymphoid tissues and testis, and it can also act as an extracellular inflammatory signal.
| Target | High mobility group protein B2 (gene HMGB2; UniProt P26583, human) |
|---|---|
| Clone | ACCF-8 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 24.0 kDa |
| Observed MW | 24 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
Samples with a confirmed band (WB): HeLa cells, PC-12 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human HMGB2.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes high mobility group protein B2 as secreted and also located in the nucleus, chromosome and cytoplasm. Tissue expression noted by UniProt (human): Expressed in gastric and intestinal tissues (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
