| Field | Specification |
|---|---|
| Target | |
| Alternative names | Hydroxymethylglutaryl-CoA synthase, mitochondrial; HMG-CoA synthase; 3-hydroxy-3-methylglutaryl coenzyme A synthase; HMGCS2 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human HMGCS2 recombinant protein (Position: M1-P487). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects hydroxymethylglutaryl-CoA synthase, mitochondrial (gene HMGCS2) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 56.6 kDa, and the supplier reports an observed band at about 50 kDa.
Mitochondrial HMG-CoA synthase (HMGCS2) makes HMG-CoA from acetyl-CoA and acetoacetyl-CoA inside mitochondria, the committed step of ketone body production. It is expressed mainly in liver and is strongly induced by fasting through PPARα, so it is a marker in studies of ketogenesis and fatty acid metabolism.
| Target | Hydroxymethylglutaryl-CoA synthase, mitochondrial (gene HMGCS2; UniProt P54868, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 56.6 kDa |
| Observed MW | 50 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human Caco-2 cells, rat liver tissue, rat kidney tissue, mouse RH35 cells, mouse liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.25 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human gastric cancer tissue, human laryngeal squamous cells carcinoma tissue, human liver cancer tissue, human gall bladder adenosquamous carcinoma tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): Caco-2 cells.
Samples with a confirmed signal (flow cytometry): Caco-2 cells.
Immunogen
Recombinant human HMGCS2 fragment (Met1–Pro487), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places hydroxymethylglutaryl-CoA synthase, mitochondrial in the mitochondrion. Tissue expression noted by UniProt (human): Expression in liver is 200-fold higher than in any other tissue. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Feng-Ming Tian et al. (2023) A UPLC-Q-TOF/MS and network pharmacology method to explore the mechanism of Anhua fuzhuan tea intervention in nonalcoholic fatty liver disease. Food & Function. 10.1039/D2FO02774G
Ningning Chen et al. (2023) Lysine β-hydroxybutyrylation promotes lipid accumulation in alcoholic liver disease. Biochemical Pharmacology. 10.1016/j.bcp.2023.115936
Chahua Huang et al. (2026) SIRT5 Inhibits HMGCS2 Succinylation and Promotes Its PIAS4-Dependent Ubiquitination to Attenuate Diabetic Cardiomyopathy. FASEB Journal. 10.1096/fj.202502832R
