| Field | Specification |
|---|---|
| Target | |
| Alternative names | Hydroxymethylglutaryl-CoA synthase, mitochondrial; HMG-CoA synthase; 3-hydroxy-3-methylglutaryl coenzyme A synthase; HMGCS2 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human HMGCS2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 25H51 is a rabbit monoclonal antibody that recognizes hydroxymethylglutaryl-CoA synthase, mitochondrial (gene HMGCS2) in human, mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 56.6 kDa, and the supplier reports an observed band at about 50 kDa.
Mitochondrial HMG-CoA synthase (HMGCS2) makes HMG-CoA from acetyl-CoA and acetoacetyl-CoA inside mitochondria, the committed step of ketone body production. It is expressed mainly in liver and is strongly induced by fasting through PPARα, so it is a marker in studies of ketogenesis and fatty acid metabolism.
| Target | Hydroxymethylglutaryl-CoA synthase, mitochondrial (gene HMGCS2; UniProt P54868, human) |
|---|---|
| Clone | 25H51 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 56.6 kDa |
| Observed MW | 50 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): human RT4 cells, human Caco-2 cells, rat liver tissue, rat kidney tissue, mouse liver tissue, mouse kidney tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human colon cancer tissue, human liver cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human HMGCS2.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places hydroxymethylglutaryl-CoA synthase, mitochondrial in the mitochondrion. Tissue expression noted by UniProt (human): Expression in liver is 200-fold higher than in any other tissue. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
