| Field | Specification |
|---|---|
| Target | |
| Alternative names | Non-histone chromosomal protein HMG-17; High mobility group nucleosome-binding domain-containing protein 2; HMGN2; HMG17 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human HMGN2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 25H24 is a rabbit monoclonal antibody that recognizes non-histone chromosomal protein HMG-17 (gene HMGN2) in human samples and is validated for Western blot and IHC. The predicted molecular weight is 9.4 kDa, and the supplier reports an observed band at about 18 kDa.
HMGN2 (HMG-17) is a small nucleosome-binding protein that attaches to nucleosome core particles and loosens chromatin compaction. By changing how DNA interacts with the histone core it helps keep genes accessible for transcription, and it is studied in chromatin structure and gene regulation.
| Target | Non-histone chromosomal protein HMG-17 (gene HMGN2; UniProt P05204, human) |
|---|---|
| Clone | 25H24 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 9.4 kDa |
| Observed MW | 18 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): K562 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human HMGN2.
Reactivity Notes
The supplier lists reactivity with human. UniProt places non-histone chromosomal protein HMG-17 in the nucleus and cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
