| Field | Specification |
|---|---|
| Target | |
| Alternative names | Heterogeneous nuclear ribonucleoproteins C1/C2;hnRNP C1/C2;HNRNPC;HNRPC; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human hnRNP C1/C2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone FEB-8 is a rabbit monoclonal antibody that recognizes heterogeneous nuclear ribonucleoproteins C1/C2 (gene HNRNPC) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 33.7 kDa, and the supplier reports an observed band at about 40 kDa.
hnRNP C1/C2 (HNRNPC) is a core component of 40S hnRNP particles that coat newly made pre-mRNA. It binds uridine-rich stretches, influences splicing, mRNA stability and translation, and its binding is enhanced where m6A modification loosens RNA structure, making it a subject in RNA processing and epitranscriptomics research.
| Target | Heterogeneous nuclear ribonucleoproteins C1/C2 (gene HNRNPC; UniProt P07910, human) |
|---|---|
| Clone | FEB-8 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 33.7 kDa |
| Observed MW | 40 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:1000–1:5000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): human HeLa cells, human HepG2 cells, human K562 cells, human Jurkat cells, rat brain tissue, rat RH35 cells, mouse brain tissue, mouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:1000 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human lymphoma tissue, human ovarian cancer tissue, human esophageal cancer tissue, human rectal cancer tissue, human breast cancer tissue, human liver cancer tissue, mouse brain tissue, rat brain tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human hnRNP C1/C2.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places heterogeneous nuclear ribonucleoproteins C1/C2 in the nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
