Anti-HOXA9 Rabbit Monoclonal Antibody

SKU:BHA21010067
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Boster Bio
Boster Bio
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Overview
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Anti-HOXA9 antibody (Rabbit monoclonal, clone 25H74, IgG). Validated for Western blot. Reactivity: Human. Recognizes homeobox protein Hox-A9. Observed band in Western blot: about 35 kDa.
Target HOXA9
clone number 25H74
Host Rabbit
Reactivity Human
Isotype IgG
Application(s) WB
Options selector
Catalog no. Size Conjugation
M00912 100 uL/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • Size: 100 uL/vial; Conjugation: Unconjugated
      Form: Liquid
      Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
      Applications: WB
      Application details: WB 1:500-2000<br>
      Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
  • Lead time: typically ships in ~2-3 business days; timing may vary by selected option.
  • Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target HOXA9
Alternative names Homeobox protein Hox-A9; Homeobox protein Hox-1G; HOXA9; HOX1G
UniProt # P31269
Host Rabbit
Clone 25H74
Clonality
  • Monoclonal
Isotype
  • IgG
Reactivity
  • Human
Applications
  • Western Blot
Immunogen A synthesized peptide derived from human HOXA9
Molecular weight 30.2 kDa
Purification Affinity-chromatography
Reconstitution Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Cellular localization Nucleus. Cytoplasm.
Concentration 0.5mg/ml
Form Liquid
Storage Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) M00912
Main SKU BHA21010067
Rabbit Monoclonal

Product Overview

Clone 25H74 is a rabbit monoclonal antibody that recognizes homeobox protein Hox-A9 (gene HOXA9) in human samples and is validated for Western blot. The predicted molecular weight is 30.2 kDa, and the supplier reports an observed band at about 35 kDa.

HOXA9 is a homeobox transcription factor that, like other HOX proteins, helps set positional identity in the embryo. In adults it is highly expressed in hematopoietic stem and progenitor cells, where it supports self-renewal. Its overexpression, often with MEIS1, is a hallmark of MLL-rearranged and other acute leukemias, and it also regulates endothelial genes.

Target Homeobox protein Hox-A9 (gene HOXA9; UniProt P31269, human)
Clone 25H74
Host / Clonality / Isotype Rabbit / Monoclonal / IgG
Reactivity Human
Form Liquid
Formulation PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers
Calculated MW 30.2 kDa
Observed MW 35 kDa
Storage Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Validated Applications

Western blot 1:500–1:2000

Immunogen

Synthetic peptide based on the sequence of human HOXA9.

Reactivity Notes

The supplier lists reactivity with human. UniProt places homeobox protein Hox-A9 in the nucleus and cytoplasm.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: Western blot 1:500–1:2000. Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Human. Other species are not listed by the supplier.
Q.What band size should be expected in Western blot?
A.Reported by the supplier: calculated molecular weight 30.2 kDa; observed band at about 35 kDa. A difference from the calculated size is common and can reflect post-translational modification, processing or isoform differences.
Q.What immunogen was used to raise this antibody?
A.Synthetic peptide based on the sequence of human HOXA9.
Q.How should this antibody be stored?
A.Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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